Mike Marko
Wadsworth Center, Albany
A survey of electron microscopic methods for 3-D imaging
The earliest method of 3-D imaging in TEM was stereoscopy, and surface topology has long been studied by SEM. The dual-beam FIB-SEM facilitates 3-D reconstruction by successive ablation and imaging. Electron tomography from a tilt series of TEM images is effective in study of structures too complex to be understood by stereoscopy, and is applied widely in both material and biological sciences. A related TEM method is used to reconstruct macromolecules at near-atomic resolution. A form of electron tomography based on imaging depth planes in STEM mode is capable of 3-D resolution on the atomic scale. Elemental analysis by EELS or EDS can be employed in 3-D, either with tilt-series TEM tomography or depth-series FIB tomography. Each of these techniques will be briefly explained and illustrated by application examples.
$5 for paid-up CDMMS members, $10 for non-members.
RSVP to Laurie LeTarte, letarte@crd.ge.com by May 8
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